Estimation of postmortem interval using top-down HPLC–MS analysis of peptide fragments in vitreous humour: A pilot study

M. Boroumand; V. M. Grassi; F. Castagnola; Fabio De Giorgio; E. d'Aloja; Giuseppe Vetrugno; Vincenzo Lorenzo Pascali; F. Vincenzoni; Federica Iavarone; G. Faa; M. Castagnola

doi:10.1016/j.ijms.2022.116952

Estimation of postmortem interval using top-down HPLC–MS analysis of peptide fragments in vitreous humour: A pilot study

M. Boroumand, V. M. Grassi, F. Castagnola, Fabio De Giorgio^*, E. d'Aloja, Giuseppe Vetrugno, Vincenzo Lorenzo Pascali^*, F. Vincenzoni, Federica Iavarone, G. Faa, M. Castagnola

^*Autore corrispondente per questo lavoro

Risultato della ricerca: Contributo in rivista › Articolo in rivista

Abstract

This study reports the detection of a set of 35 peptide fragments and 7 intact proteins in the vitreous humour using a top-down proteomic platform based on high-resolution HPLC-MS and MS/MS analysis. The concentrations of thymosin b4 (R = 0.932) and two peptide fragments (i.e., vimentin fragment (Fr.) 443-465 (R = 0.998) and polyubiquitin Fr. 1-73 (R = 0.968)) were observed to have very strong linear correlations with postmortem intervals. Data are available via ProteomeXchange with identifier PXD037095. These preliminary results suggest that some biochemical molecular events are linearly related to the postmortem interval and that the concentrations of these peptides and fragments can be clinically useful in establishing the time of death if measured within the first 160 h postmortem. (c) 2022 Elsevier B.V. All rights reserved.

Lingua originale	English
pagine (da-a)	N/A-N/A
Rivista	International Journal of Mass Spectrometry
Volume	483
Numero di pubblicazione	5
DOI	https://doi.org/10.1016/j.ijms.2022.116952
Stato di pubblicazione	Pubblicato - 2023

All Science Journal Classification (ASJC) codes

Instrumentation
Condensed Matter Physics
Spectroscopy
Physical and Theoretical Chemistry

Keywords

Forensic autopsy
Peptide fragments
Postmortem interval
Postmortem investigation
Time of death
Top-down HPLC-MS analysis
Vitreous humour

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10.1016/j.ijms.2022.116952

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Boroumand, M., Grassi, V. M., Castagnola, F., De Giorgio, F., d'Aloja, E., Vetrugno, G., Pascali, V. L., Vincenzoni, F., Iavarone, F., Faa, G., & Castagnola, M. (2023). Estimation of postmortem interval using top-down HPLC–MS analysis of peptide fragments in vitreous humour: A pilot study. International Journal of Mass Spectrometry, 483(5), N/A-N/A. https://doi.org/10.1016/j.ijms.2022.116952

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abstract = "This study reports the detection of a set of 35 peptide fragments and 7 intact proteins in the vitreous humour using a top-down proteomic platform based on high-resolution HPLC-MS and MS/MS analysis. The concentrations of thymosin b4 (R = 0.932) and two peptide fragments (i.e., vimentin fragment (Fr.) 443-465 (R = 0.998) and polyubiquitin Fr. 1-73 (R = 0.968)) were observed to have very strong linear correlations with postmortem intervals. Data are available via ProteomeXchange with identifier PXD037095. These preliminary results suggest that some biochemical molecular events are linearly related to the postmortem interval and that the concentrations of these peptides and fragments can be clinically useful in establishing the time of death if measured within the first 160 h postmortem. (c) 2022 Elsevier B.V. All rights reserved.",

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author = "M. Boroumand and Grassi, {V. M.} and F. Castagnola and {De Giorgio}, Fabio and E. d'Aloja and Giuseppe Vetrugno and Pascali, {Vincenzo Lorenzo} and F. Vincenzoni and Federica Iavarone and G. Faa and M. Castagnola",

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Boroumand, M, Grassi, VM, Castagnola, F, De Giorgio, F, d'Aloja, E, Vetrugno, G , Pascali, VL, Vincenzoni, F, Iavarone, F, Faa, G & Castagnola, M 2023, 'Estimation of postmortem interval using top-down HPLC–MS analysis of peptide fragments in vitreous humour: A pilot study', International Journal of Mass Spectrometry, vol. 483, n. 5, pagg. N/A-N/A. https://doi.org/10.1016/j.ijms.2022.116952

TY - JOUR

T1 - Estimation of postmortem interval using top-down HPLC–MS analysis of peptide fragments in vitreous humour: A pilot study

AU - Boroumand, M.

AU - Grassi, V. M.

AU - Castagnola, F.

AU - De Giorgio, Fabio

AU - d'Aloja, E.

AU - Vetrugno, Giuseppe

AU - Pascali, Vincenzo Lorenzo

AU - Vincenzoni, F.

AU - Iavarone, Federica

AU - Faa, G.

AU - Castagnola, M.

PY - 2023

Y1 - 2023

N2 - This study reports the detection of a set of 35 peptide fragments and 7 intact proteins in the vitreous humour using a top-down proteomic platform based on high-resolution HPLC-MS and MS/MS analysis. The concentrations of thymosin b4 (R = 0.932) and two peptide fragments (i.e., vimentin fragment (Fr.) 443-465 (R = 0.998) and polyubiquitin Fr. 1-73 (R = 0.968)) were observed to have very strong linear correlations with postmortem intervals. Data are available via ProteomeXchange with identifier PXD037095. These preliminary results suggest that some biochemical molecular events are linearly related to the postmortem interval and that the concentrations of these peptides and fragments can be clinically useful in establishing the time of death if measured within the first 160 h postmortem. (c) 2022 Elsevier B.V. All rights reserved.

AB - This study reports the detection of a set of 35 peptide fragments and 7 intact proteins in the vitreous humour using a top-down proteomic platform based on high-resolution HPLC-MS and MS/MS analysis. The concentrations of thymosin b4 (R = 0.932) and two peptide fragments (i.e., vimentin fragment (Fr.) 443-465 (R = 0.998) and polyubiquitin Fr. 1-73 (R = 0.968)) were observed to have very strong linear correlations with postmortem intervals. Data are available via ProteomeXchange with identifier PXD037095. These preliminary results suggest that some biochemical molecular events are linearly related to the postmortem interval and that the concentrations of these peptides and fragments can be clinically useful in establishing the time of death if measured within the first 160 h postmortem. (c) 2022 Elsevier B.V. All rights reserved.

KW - Forensic autopsy

KW - Peptide fragments

KW - Postmortem interval

KW - Postmortem investigation

KW - Time of death

KW - Top-down HPLC-MS analysis

KW - Vitreous humour

KW - Forensic autopsy

KW - Peptide fragments

KW - Postmortem interval

KW - Postmortem investigation

KW - Time of death

KW - Top-down HPLC-MS analysis

KW - Vitreous humour

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SN - 1387-3806

VL - 483

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JO - International Journal of Mass Spectrometry

JF - International Journal of Mass Spectrometry

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ER -

Estimation of postmortem interval using top-down HPLC–MS analysis of peptide fragments in vitreous humour: A pilot study

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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